A rapid assay method for hyaluronidase
โ Scribed by C.J. Coulson; R. Girkin
- Book ID
- 107711748
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 416 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A spectrophotometric method for the assay of hyaluronidase activity, based on the binding of a carbocyanine dye (l-ethyl-2-[3-( l-ethyl-naphtho[l,M] to undegraded substrate, is described. The binding results in a spectral shift with an absorbance maximum at 640 nm which is propo~ional to the amount
A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein