A simple and rapid method for isolation of cellular DNA
β Scribed by N. B. Ramachandra
- Publisher
- Indian Academy of Sciences
- Year
- 1998
- Tongue
- English
- Weight
- 173 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0971-8044
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 an
A rapid method is described for the isolation of plasmid DNA from Escherichia coli and Pseudomonas putida. The effect of heating the cell preparation during plasmid extraction is discussed in relationship to the final plasmid yield.
## Abstract Various pathological conditions are associated with changes in multiple protein biomarkers, and these changes can be assessed using xMAP^β’^ beads and the Luminex^β’^ platform. Although this platform is most commonly utilized in the analysis of biomarkers in serum, plasma, or urine, it is
A new method has been developed for determination of DNA synthesis during cell proliferation. The method is based on the metabolism of [U-13 C 6 ]glucose to deoxyribose (DR) and then incorporation of [U-13 C 5 ]DR into newly synthesized DNA. Extracted cellular DNA is subjected to HCl hydrolysis (2 h