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A simple, rapid, and convenient Luminex™-compatible method of tissue isolation

✍ Scribed by Derek Barclay; Ruben Zamora; Andres Torres; Rajaie Namas; David Steed; Yoram Vodovotz

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
136 KB
Volume
22
Category
Article
ISSN
0887-8013

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Various pathological conditions are associated with changes in multiple protein biomarkers, and these changes can be assessed using xMAP^™^ beads and the Luminex^™^ platform. Although this platform is most commonly utilized in the analysis of biomarkers in serum, plasma, or urine, it is often desirable to measure these analytes in tissues (e.g., biopsy specimens). We have developed a simple, rapid method of tissue isolation in which excised tissues are permeabilized in RNAlater^™^ at 4°C overnight, followed by Luminex^™^ analysis for inflammatory biomarkers (cytokines). This method was compared with flash‐freezing in both mouse liver and human debrided wound specimens, and may be of utility in other clinical settings. J. Clin. Lab. Anal. 22:278–281,
2008. © 2008 Wiley‐Liss, Inc.

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