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A Simple and Efficient Method for the Purification of Membrane-Bound Levansucrase fromZymomonas mobilis

✍ Scribed by Armands Vigants, Hans-Georg Hicke, Stefan Peter Marx

Book ID
120778175
Publisher
Springer
Year
2001
Tongue
English
Weight
329 KB
Volume
42
Category
Article
ISSN
0343-8651

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An easy separation of bacterial cells from the desired exopolysaccharide with an important reduction in protein content is achieved by adjusting the pH of the culture broth to 4 to 5 before autoclaving. There is a drastic reduction in the apparent viscosity, with formation of a cell aggregate which