A simple and efficient method for purification of prawn baculovirus DNA
โ Scribed by F Yang; W Wang; R.Z Chen; X Xu
- Book ID
- 114140400
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 239 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0166-0934
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## Abstract Baculovirus has emerged as a novel gene delivery and vaccine vector, and the demand for purified baculovirus is rising due to the increasing in vivo applications. Since the baculoviral envelope protein gp64 is a glycoprotein, we aimed to develop a concanavalin A (Con A) chromatography p
## SUMMPARY A simple, efficient method was used to purify QCR primers which had degraded during storage at -2O"C/-9O'C. The primers were electrophoresed ou 3 A (w/v) agarose gel, the main band was electroeluted via a trough cut in the gel. The primers were recovered by isobutanol extraction follo