𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple and efficient method for the purification of an exopolysaccharide fromKlebsiellasp. I-714

✍ Scribed by J. Farrés; G. Caminal; J. López-Santín

Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
384 KB
Volume
10
Category
Article
ISSN
0951-208X

No coin nor oath required. For personal study only.

✦ Synopsis

An easy separation of bacterial cells from the desired exopolysaccharide with an important reduction in protein content is achieved by adjusting the pH of the culture broth to 4 to 5 before autoclaving. There is a drastic reduction in the apparent viscosity, with formation of a cell aggregate which is easily sedimented. The resulting solution contains all the exopolysaccharide (the free, and the linked one), the amount of protein being reduced by 40%.

📜 SIMILAR VOLUMES

A Simple Method for the Purification of
A Simple Method for the Purification of Type 1 RIPs
✍ Giordana Marcozzi; Laura Spano 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 106 KB 👁 2 views

We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.