✦ LIBER ✦

A reliable analysis of tissue free fatty acids by gas-liquid chromatography

✍ Scribed by Kouji Kishiro; Hiroshi Yasuda

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 358 KB
Volume: 175
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90576-3

No coin nor oath required. For personal study only.

✦ Synopsis

A convenient and reliable gas-liquid chromatographic method for determining the free fatty acids in biological specimens is described. The free fatty acids were extracted with hexane in the presence of H3PO4 and then back-extracted from the hexane phase into a very small volume of trimethyl (alpha, alpha, alpha-trifluoro-m-tolyl)ammonium hydroxide solution. Direct injection of the resultant quaternary ammonium salts of the fatty acids into a gas-liquid chromatograph unit gave their methyl esters, with a high recovery. The presence of triglycerides, phospholipids, or cholesterol esters did not interfere with the determination of free fatty acids. This method was applied to determination of free fatty acids in the samples of serum or brain. The results were more precise and reliable than those reported with the conventional methods with TLC separation. This method should be a useful aid for providing precise information about the physiological or pathological roles of free fatty acids.

📜 SIMILAR VOLUMES

Quantitative analysis of ascorbic acid i

Quantitative analysis of ascorbic acid in tissues by gas-liquid chromatography

✍ J.H. Allison; M.A. Stewart 📂 Article 📅 1971 🏛 Elsevier Science 🌐 English ⚖ 499 KB

Several investigators have reported that ascorbic acid can be analyzed by gas-liquid chromatography (GLC) following conversion of the parent compound to its trimethylsilyl ether (l-5). We have applied this procedure to the analysis of ascorbic acid in tissues and in this report describe the techniqu

The analysis of polyunsaturated fatty ac

The analysis of polyunsaturated fatty acids in meat by capillary gas-liquid chromatography

✍ Andrew J. Sinclair; William J. Slattery; Kerin O'Dea 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 349 KB 👁 2 views

Cyclopropenoid Fatty Acids of Sterculia

Cyclopropenoid Fatty Acids of Sterculia Oils by Gas Liquid Chromatography

✍ Badami, R. C. ;Patil, K. B. ;Subbarao, Y. V. ;Sastri, G. S. R. ;Vishvanathrao, G 📂 Article 📅 1980 🏛 John Wiley and Sons ⚖ 228 KB

## Abstract Silver nitrate treated methyl esters of the seed oils of Sterculia alata, Sterculia foetida, Sterculia guttata and Sterculia villosa were subjected to gas liquid chromatography and were found to contain sterculic and malvalic acids (4.0, 17.6; 55.7, 11.4; 5.8, 2.1 and 3.2, 2.5%, respect

Cyclopropenoic Fatty Acids of Malvaceae

Cyclopropenoic Fatty Acids of Malvaceae Seed Oils by Gas-Liquid Chromatography

✍ Gaydou, E. M. ;Ramanoelina, A. R. P. 📂 Article 📅 1984 🏛 John Wiley and Sons ⚖ 330 KB

I andern, fur welche die Kugeldruckharte anstelle dGr NPZ die geeignetere Methode darste

Quantitative analysis of fecal bile acid

Quantitative analysis of fecal bile acids by gas-liquid chromatography

✍ M. Makita; W.W. Wells 📂 Article 📅 1963 🏛 Elsevier Science 🌐 English ⚖ 452 KB

The analysis of bile acids by spectrophotometric methods is often associated with significant errors from contaminating compounds (1, 2). Furthermore, the response of dihydroxy-and trihydroxycholanic acids to various calorimetric and spectrophotometric methods is variable (3). Partition chromatograp

Analysis of sucrose fatty acid esters by

Analysis of sucrose fatty acid esters by high temperature gas chromatography

✍ Karrer, Randolf ;Herberg, Hanna 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 405 KB 👁 1 views

## Abstract A commercially available high temperature GC system has been investigated to determine its suitability for the automated analysis of sucrose fatty acid esters. In comparison with conventional GC, the high temperature variety broadens the scope of the technique to encompass high molecula