Quantitative analysis of fecal bile acids by gas-liquid chromatography

✍ Scribed by M. Makita; W.W. Wells

• Publisher: Elsevier Science
• Year: 1963
• Tongue: English
• Weight: 452 KB
• Volume: 5
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(63)90072-1

✦ Synopsis

The analysis of bile acids by spectrophotometric methods is often associated with significant errors from contaminating compounds (1, 2). Furthermore, the response of dihydroxy-and trihydroxycholanic acids to various calorimetric and spectrophotometric methods is variable (3). Partition chromatography procedures (4) are lengthy and, in the case of fecal extracts, may be hindered by the presence of microbial hydroxy fatty acids (5).

Vanden Heuvel et al. ( 6) reported that gas-liquid chromatography was useful in the separation of bile acid methyl esters and Blomstrand (7) subjected the methyl esters of human biliary bile acids to gas-liquid chromatography.

Sjiivall et al. ( 8) have reported the chromatography of substituted cholanic acids on polar phenyl silicone phases, and Holmes et al. ( 9) and Bloomfield (10) have studied model conditions for bile acid analysis by gas-liquid chromatography.

In connection with our studies of cholesterol metabolism, a method for the quantitative analysis of fecal neutral sterol trimethylsilyl ethers on a polar nitryl silicone liquid phase was reported (11). Gas chromatographic separation of the trimethylsilyl ethers of a wide variety of methyl cholanates on the nitryl silicone phase used in the former study was found to be inadequate. Polar phases (e.g., ethylene glycol polysuccinate), commonly employed for fatty acid methyl esters, were unstable at temperatures required to elute the methyl cholanate trimethylsilyl ethers. However, a polar phase, Hi Eff-8B,4 which was stable up to 280°C was successful in separating the major fecal bile acids. This re-