Male Wistar rats weighing 200-250 g were used. I-d,-Estrtrtliol. To a solution of 2-iodoestradiol (16) (IO mg) and PdCl, (10 mg) dissolved in methanol (0.7 ml)-D,O (0.1 ml) was added NaBD, (2 mg), and the resulting solution was stirred at 0ยฐC for 40 min under a stream of nitrogen gas. The reaction
A rapid simple radiometric assay for phospholipase A2 activity
โ Scribed by M.James Cosentino; LeGrande C. Ellis
- Book ID
- 119049105
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 526 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0090-6980
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Phospholipase A 2 (PLA 2 ) is an enzyme that cleaves fatty acids from the sn-2 position of membrane phospholipids. Intracellular PLA 2 has been shown to be important for the generation of arachidonic acid, which leads to the synthesis of a wide range of eicosanoid paracrine hormones. Furthermore, se
A rapid and simple radiometric assay for cholinesterase, suitable for multiple determinations, has been developed. (aH-acetyl) choline is enzymatically hydrolyzed in a small reaction volume in a scintillation vial. The released [all]acetate is then extracted into a toluene-based scintillator added d