𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A rapid, sensitive assay for glycine-directed amidating enzymes

✍ Scribed by L.J. Moray; Clara R. Miller; Walker H. Busby Jr.; Jane Humm; Robert C. Bateman Jr.; John S. Kizer


Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
381 KB
Volume
14
Category
Article
ISSN
0165-0270

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


A rapid and sensitive assay for arginase
✍ Urs T. RΓΌegg; Anthony S. Russell πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 516 KB

An assay for arginase is described that uses L-[guanido-'"Clarginine as substrate. Unhydrolyzed arginine is removed in a batch procedure with sulfonate resin and the ['"Clurea product is determined quantitatively in the resin supernatant. The assay requires 5 min and is performed in one tube. The se

A rapid assay for the glyoxalase enzyme
✍ Nicholas M. Alexander; James L. Boyer πŸ“‚ Article πŸ“… 1971 πŸ› Elsevier Science 🌐 English βš– 541 KB

The glyoxalase enzyme system was first detected in animal tissues in 1913 (1,2). It is widely distributed in nature (3-6) and consists of two enzymes, glyoxalase I (S-lactoyl-glutathione methylglyoxal-lyase (isomerizing) , EC 4.4.1.5) and glyoxalase II (S-2-hydroxyacrylglutathione hydrolase, EC 3.1.

A rapid and sensitive assay method for p
✍ Kuo-Ping Huang; J.C. Robinson πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 421 KB

A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein

A rapid method for the quantitative assa
✍ Walter L. Nelson; Edward I. Ciaccio; George P. Hess πŸ“‚ Article πŸ“… 1961 πŸ› Elsevier Science 🌐 English βš– 325 KB

Insoluble dye-protein complexes offer many advantages when used as substrates for proteolytie enzymes. The proteolytic split products can be separated from the starting substrat.e conveniently by filtration, and the concentration of the soluble colored products of hydrolysis can easily be measured a