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A rapid assay for the glyoxalase enzyme system

✍ Scribed by Nicholas M. Alexander; James L. Boyer

Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
541 KB
Volume
41
Category
Article
ISSN
0003-2697

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✦ Synopsis

The glyoxalase enzyme system was first detected in animal tissues in 1913 (1,2). It is widely distributed in nature (3-6) and consists of two enzymes, glyoxalase I (S-lactoyl-glutathione methylglyoxal-lyase (isomerizing) , EC 4.4.1.5) and glyoxalase II (S-2-hydroxyacrylglutathione hydrolase, EC 3.1.2.6), plus the cofactor reduced gluthathione (GSH). This enzyme system catalyzes the conversion of some ketoaldehydes to hydroxy acids. For example, methylglyoxal is converted to lactic acid in a two-step reaction in the following manner:

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