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A rapid method for preparation of bacterial plasmids

✍ Scribed by James Summerton; Thomas Atkins; Richard Bestwick

Book ID
107711829
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
831 KB
Volume
133
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

A rapid boiling method for the preparati
A rapid boiling method for the preparation of bacterial plasmids
✍ David S. Holmes; Michael Quigley πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 1005 KB

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca

A note on the use of CsCl centrifugation
A note on the use of CsCl centrifugation to purify bacterial plasmids prepared by the rapid boiling method
✍ A.J. Eisenberg; David S. Holmes πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 83 KB

A modification of the rapid boiling method for the preparation of bacterial plasmids allows it to be readily used in conjunction with standard CsCI-ethidium bromide density centrifugation for the-isolation of purified plasmids.