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A rapid method for identifying plasmids in cyanobacteria

✍ Scribed by Kim Stutzman Engwall; Steve M. Gendel

Book ID
109313144
Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
431 KB
Volume
26
Category
Article
ISSN
0378-1097

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Fifteen strains of cyanobacteria, mainly Nostoc and Anabaena species, were screened for plasmids using five rapid procedures. Two of these methods, based on alkaline extraction and phenol extraction of cleared lysates respectively, were successful with a total of ten species, the latter method provi

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A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca