A method for enzymatic glycogen determination in yeast cells without need for prior disruption of the cell walls has been developed: After stopping cell metabolism with 5% trichloroacetic acid, the cells were digested with 0.25 M Na&O, for 90 min on a boiling water bath and pH was adjusted to 4.8. G
β¦ LIBER β¦
A rapid method for determination of acid phosphatase activity of whole yeast cells
β Scribed by D. Galabova; B. Tuleva; M. Balasheva
- Book ID
- 115272044
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 189 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0266-8254
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method for glycogen determination in w
β
JΓΆrn Ullrich Becker
π
Article
π
1978
π
Elsevier Science
π
English
β 560 KB
Determination of Acid Phosphatase Activi
β
ESPOSTI, P. L.; ESTBORN, B.; ZAJICEK, J.
π
Article
π
1960
π
Nature Publishing Group
π
English
β 223 KB
A rapid permeabilization procedure for a
β
Fred Kippert
π
Article
π
1995
π
John Wiley and Sons
π
English
β 456 KB
Multiwell Filtration Assay for Rapid Det
β
R. Gopalakrishna; U. Gundimeda; J.C. Wilson; Z.H. Chen
π
Article
π
1993
π
Elsevier Science
π
English
β 413 KB
A spectrophotometric method for the dete
β
P. Gierow; B. Jergil
π
Article
π
1980
π
Elsevier Science
π
English
β 361 KB
A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine
Use of phenolphthalein diphosphate in th
β
G.W.F.H. Borst Pauwels
π
Article
π
1965
π
Elsevier Science
π
English
β 457 KB