Multiwell Filtration Assay for Rapid Determination of Protein Phosphatase Activity
โ Scribed by R. Gopalakrishna; U. Gundimeda; J.C. Wilson; Z.H. Chen
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 413 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An assay was developed for carbamyl phosphate phosphatase (CAP phosphatase) activity. This enzyme activity is difficult to assay because of the chemical instability of carbamyl phosphate (CAP). We chose to measure CAP phosphatase activity by monitoring release of inorganic phosphate (Pi). This entai
Despite the physiological importance of serine/threonine protein phosphatase type 2B (PP2B/calcineurin), an accurate assay method of PP2B in crude tissue extracts has not been established. By using recombinant protein phosphatase inhibitor-1 as a substrate and ascorbic acid as an antioxidant, we dev