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A rapid and versatile site-directed method of mutagenesis for double-stranded plasmid DNA

โœ Scribed by Bellini, Ada Velati; de Ferra, Francesca; Grandi, Guido


Book ID
122592169
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
700 KB
Volume
69
Category
Article
ISSN
0378-1119

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Site-directed mutagenesis by complementa
โœ Steve N. Slilaty; Margaret Fung; Shi-Hsiang Shen; Susan Lebel ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 767 KB

An approach for generating structures capable of directing full-length complementary-strand synthesis for double-stranded plasmid DNA is described. The structures are formed following heat denaturation and cooling of linearized plasmid DNA molecules in the presence of what is referred to as a "closi