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Rapid and economical multiple base site-directed mutagenesis of double stranded plasmid DNA

✍ Scribed by Kyung-Soo Kim; Charles K. Pallaghy


Book ID
110371811
Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
455 KB
Volume
11
Category
Article
ISSN
0951-208X

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Site-directed mutagenesis by complementa
✍ Steve N. Slilaty; Margaret Fung; Shi-Hsiang Shen; Susan Lebel πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 767 KB

An approach for generating structures capable of directing full-length complementary-strand synthesis for double-stranded plasmid DNA is described. The structures are formed following heat denaturation and cooling of linearized plasmid DNA molecules in the presence of what is referred to as a "closi