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A simple method for site-directed mutagenesis with double-stranded plasmid DNA

✍ Scribed by Derhsing Lai, Xuli Zhu, Sidney Pestka

Book ID
118294321
Publisher
Humana Press Inc
Year
1998
Tongue
English
Weight
665 KB
Volume
9
Category
Article
ISSN
1073-6085

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An approach for generating structures capable of directing full-length complementary-strand synthesis for double-stranded plasmid DNA is described. The structures are formed following heat denaturation and cooling of linearized plasmid DNA molecules in the presence of what is referred to as a "closi