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A simple method for screening bacterial colonies for mutagenized sites in plasmid DNA

✍ Scribed by Juan Carlos Rodrı́guez-Lecompte; Malliga N Nagarajan; Frederick S.B Kibenge

Book ID
114139759
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
187 KB
Volume
99
Category
Article
ISSN
0166-0934

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A method for the deletion of restriction
A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid
✍ Covey, Curt ;Richardson, Denise ;Carbon, John 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 423 KB

A general method has been developed for the deletion of restriction endonuclease sites in bacterial plasmid DNA. The procedure involves partial digestion of the covalently closed circular plasmid DNA with an appropriate restriction endonuclease under conditions which allow accumulation of unit-lengt