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A method for the site-directed mono- and multi-mutagenesis of double-stranded DNA

โœ Scribed by Weiner, Michael P.; Felts, Katherine A.; Simcox, Timothy G.; Braman, Jeffrey C.


Book ID
123286574
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
813 KB
Volume
126
Category
Article
ISSN
0378-1119

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An approach for generating structures capable of directing full-length complementary-strand synthesis for double-stranded plasmid DNA is described. The structures are formed following heat denaturation and cooling of linearized plasmid DNA molecules in the presence of what is referred to as a "closi