A simple method for purifying [14C]butyrylcholine and incubation conditions suitable for estimating butyrylcholinesterase activity are described. Solvent extraction and anion-and cation-exchange procedures for separating butyrylcholine from butyric acid are described and compared. The anion-exchange
A radiometric microassay for cellulase activity
โ Scribed by Alexander Vardanis; Malcolm Finkelman
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 210 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The method described measures the release of 14C products from [U-'4C]cellulose. The assay is simple and utilizes very small incubation volumes. Activity is evident within the first 30 min of reaction. This method should be particularly suited to activity surveys of culture filtrates from fermentations as well as the analysis of fractions from enzyme purification studies.
๐ SIMILAR VOLUMES
A sensitive microassay technique for determining IJDP-galactose 4-epimerase activity in cell-free lysates has been devised. This method measures directly the conversion of UDP-galactose to UDP-glucose by thin-layer chromatographic separation of the two compounds. Radioactive labeling of the substrat