A microassay for lysyl oxidase activity
β Scribed by Ronald L. Misiorowski; Judith B. Ulreich; Milos Chvapil
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 367 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Enzyme activity is determined by scintillation co
A simple, efficient, and rapid procedure for the purification of lysyl oxidase is described. This method utilizes an affinity scheme involving powdered elastin-hydroxyethylmethacrylate hydrogels and high-performance liquid chromatography and permits the study of enzyme from sources which contain lim
Lysyl oxidase catalyzes the final known enzymatic step required for collagen and elastin cross-linking in the biosynthesis of normal mature functional insoluble extracellular matrices. In addition, lysyl oxidase has been identified as a possible tumor suppressor. Lysyl oxidase activity in biological