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A micromethod for the purification of lysyl oxidase

✍ Scribed by Rocco Ferrera; Barbara Faris; Peter J. Mogayzel Jr.; Wayne A. Gonnerman; Carl Franzblau


Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
477 KB
Volume
126
Category
Article
ISSN
0003-2697

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✦ Synopsis


A simple, efficient, and rapid procedure for the purification of lysyl oxidase is described. This method utilizes an affinity scheme involving powdered elastin-hydroxyethylmethacrylate hydrogels and high-performance liquid chromatography and permits the study of enzyme from sources which contain limited amounts of enzyme, such as aortic smooth muscle cells in culture.


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