𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A microassay for guanosine 3′,5′-monophosphate phosphodiesterase activity

✍ Scribed by Richard Fertel; Benjamin Weiss

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
835 KB
Volume
59
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Increased activity of guanosine 3′-5′-cy
Increased activity of guanosine 3′-5′-cyclic monophosphate phosphodiesterase in the renal tissue of cirrhotic rats with ascites
✍ Paolo Angeli; Wladimiro Jiménez; Rosalia Veggian; Silvano Fasolato; Roberta Volp 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 136 KB

A possible defect of guanosine 3Ј-5Ј-cyclic monophosphate (cGMP) content in the renal tissue caused by an increased activity of cGMP phosphodiesterase (PDE) has, so far, not been evaluated in the pathogenesis of renal resistance to endogenous natriuretic peptides (ENP) in cirrhosis with ascites. To

Simultaneous isolation of adenosine 3′,5
Simultaneous isolation of adenosine 3′,5′-cyclic monophosphate (cAMP) and guanosine 3′,5′-cyclic monophosphate (cGMP) in small tissue samples
✍ C.C. Mao; A. Guidotti 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 348 KB

A column chromatogrnphic sy;itrm has been developed for isolation and purification of cAMP and cGMP simultnneously from small tissue samples. In this system, a column of neutral aluminum oxide equilibrated in Tris-HCl buffer, pH 7.5 was used to purify cyclic nucleotides from olhe~ nucleotides in non

A Nonradioactive High-Performance Liquid
A Nonradioactive High-Performance Liquid Chromatographic Microassay for Uridine 5′-Monophosphate Synthase, Orotate Phosphoribosyltransferase, and Orotidine 5′-Monophosphate Decarboxylase
✍ Jerapan Krungkrai; Nuchanat Wutipraditkul; Phisit Prapunwattana; Sudaratana R. K 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 76 KB

A novel nonradioactive, microassay method has been developed to determine simultaneously the two enzymatic activities of orotate phosphoribosyltransferase (OPRTase) and orotidine 5'-monophosphate decarboxylase (ODCase), either as a bifunctional protein (uridine 5'-monophosphate synthase, UMPS) or as

A Fluorometric Assay for Cyclic Guanosin
A Fluorometric Assay for Cyclic Guanosine 3′,5′-Monophosphate Incorporating a Sep-Pak Cartridge and Enzymatic Cycling
✍ Kazuhiko Seya; Ken-Ichi Furukawa; Shigeru Motomura 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 85 KB

We developed a sensitive and nonradioactive fluorometric assay for cyclic guanosine 3,5-monophosphate (cGMP). Guanine nucleotides except cGMP were enzymatically phosphorylated to GTP. cGMP, absorbed into a Sep-Pak amino propyl cartridge, was eluted separately from GTP. Purified cGMP was enzymaticall