Some radiometric microassays for butyrylcholinesterase

✍ Scribed by Jerome L. Maderdrut

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 656 KB
• Volume: 88
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90438-4

✦ Synopsis

A simple method for purifying [14C]butyrylcholine and incubation conditions suitable for estimating butyrylcholinesterase activity are described. Solvent extraction and anion-and cation-exchange procedures for separating butyrylcholine from butyric acid are described and compared. The anion-exchange method provides the most efficient separation of precursor from product. Blanks using the anion-exchange method average approximately 0.1% of the total radioactivity in the substrate; product recovery is quantitative. Butyrylcholinesterase activity can be measured reliably in samples of embryonic nervous system tissue having wet weights less than 5 pg.

Methods

Materials. Radioisotopes were purchased from New England Nuclear (Boston, Mass.); ion-exchange resins were purchased from Bio-Rad