Radioiodine-substituted nucleosides in "3-labeled poly(U) are rapidly deiodinated by reaction with bisuhite at near neutral pH. The deiodination reaction is prevented by annealling of the iodinated poly(U) with poly(A). The latter characteristic is the basis for an analytical protocol used to charac
A Quantitative Method to Detect RNA Editing Events
β Scribed by Hans H. Schiffer; Stephen F. Heinemann
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 89 KB
- Volume
- 276
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
vidin-HRP. The improved outcome of immunoblotting is probably a result of two separate effects: (i) improved efficacy of immuno-inert membrane blocking and (ii) the presence of Tween 20, which may aid in the removal of any residual SDS from proteins and thereby help in renaturation of proteins on the nitrocellulose (12, 13).
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