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Fast isolation of RNA to detect expression of tumor markers

✍ Scribed by Jörg A. Schenk; Timo Hillebrand; Lieselotte Lübbe; Stephan Heymann; Michael Böttger; Burkhard Micheel; Peter Bendzko

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
42 KB
Volume
11
Category
Article
ISSN
0887-8013

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✦ Synopsis

The expression status of several tumorrelated proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT-PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination. We demonstrate here a simple and fast method to isolate RNA from only 10,000 cells and applied it to the detection of CEA, c-ERB-B2, and mdr-1 as often studied models for tumor markers.

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