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A method to detect nonproline cis peptide bonds in proteins

✍ Scribed by Manfred S. Weiss; Rolf Hilgenfeld

Publisher
Wiley (John Wiley & Sons)
Year
1999
Tongue
English
Weight
88 KB
Volume
50
Category
Article
ISSN
0006-3525

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✦ Synopsis

Based on the geometrical parameters around seventeen incorrectly assigned trans conformations of peptide bonds in protein structures and their correct cis counterparts, we have devised an algorithm that is capable of detecting these sites. The algorithm was optimized to reliably find all of the seventeen test cases. It can be used to quickly scan an atomic coordinate file or the complete Brookhaven Protein Data Base for more likely candidates for non-Pro cis peptide bonds. Also, it can be of help to guide the crystallographer in intermediate stages of structure determination towards suspect areas.

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The rate-limiting step in the folding of the cis-Pro167Thr mutant of TEM-1 Ξ²-lactamase is the trans to cis isomerization of a non-proline peptide bond
✍ Marc Vanhove; Xavier Raquet; Timothy Palzkill; Roger H. Pain; Jean-Marie FrΓ¨re πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 746 KB

The stability and kinetics of unfolding and refolding of the P167T mutant of the TEM-1 p-lactamase have been investigated as a function of guanidine hydrochloride concentration. The activity of the mutant enzyme was not significantly modified, which strongly suggests that the Glu166Thr167 peptide bo