An intermediate in the ethidium bromide (EB) induced petite mutation pathway may be destabilized by daylight light to cause a reversion to the normal grande phenotype. Starved cells preincubated in the dark for up to 6 h with 100 microgram/ml EB could be reverted to grandes after one hour of light e
A protective effect of caffeine on the ethidium induced petite mutation in yeast
β Scribed by S.C. Hixon; K.L. Yielding
- Book ID
- 113190900
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 280 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0027-5107
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π SIMILAR VOLUMES
The induction of lethal events and cytoplasmic "petite" mutations in random and synchronously dividing cultures of haploid and diploid cells of Saccharomyces cerevisiae was determined after treatment with 8-Methoxypsoralen plus 365 nm light. Diploid cells were always more resistant than haploid cel
It is shown that caffeine antagonizes petite-induction with ethidium bromide under non-growth conditions when administered during or after mutagenic treatment. Caffeine itself is shown to be a petite-inducing agent when cells are grown in liquid glucose-complete-medium in the presence of the drug. A