An intermediate in the ethidium bromide (EB) induced petite mutation pathway may be destabilized by daylight light to cause a reversion to the normal grande phenotype. Starved cells preincubated in the dark for up to 6 h with 100 microgram/ml EB could be reverted to grandes after one hour of light e
A synergistic effect of ultraviolet light and ethidium bromide on petite induction in yeast
โ Scribed by S.C. Hixon; K.L. Yielding
- Book ID
- 113190496
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 292 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0027-5107
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
When growing cultures of S. cerevisiae are treated with high concentrations of ethidium bromide (greater than 50 mug/ml), three phases of petite induction may be observed: I. the majority of cells are rapidly converted to petite, II. subsequently a large proportion of cells recover the ability to fo
Treatment of haploid strains of the petite negative yeast Schizosaceharornyces pombe with ethidium bromide prior to mating with untreated cells reduces transmission ofmitochondrial markers from the treated strains. This effect is fully reversible after 20 generations of growth in drug free medium be