A major variable to consider in the production of biologicals from mammalian cell cultures is the mode of operation, be it a batch, continuous, perfusion, fed-batch or other production method. The final choice must consider a number of fundamental and economic issues. Here we present some antibody p
A practical method for rescuing desired hybridomas during monoclonal antibody production
โ Scribed by Grazyna Adamus; Z. Suzanne Zam; Scotts Emerson; Paul A. Hargrave
- Book ID
- 112799441
- Publisher
- Society for In Vitro Biology
- Year
- 1989
- Tongue
- English
- Weight
- 476 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1475-2689
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## Abstract We describe a new format for surfaceโbased fluoroimmunoassays that allows detection of biomolecule interactions without separation steps. The bioactive layer was immobilized on the surface of a glass substrate covered with silver islands that provide optical amplification of the distinc
Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical dass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 X 1 O7 cells/ml was achie