✦ LIBER ✦

A polishable amperometric biosensor for bilirubin

✍ Scribed by Joseph Wang; Mebmet Ozsoz

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 298 KB
Volume: 2
Category: Article
ISSN: 1040-0397
DOI: 10.1002/elan.1140020813

No coin nor oath required. For personal study only.

✦ Synopsis

An amperometric enzyme electrode for bilirubin based on the incorporation of bilirubin oxidase and horseradish peroxidase within a graphite-epoxy matrix is reported. The resulting bienzyme electrodes are renewable (by polishing) and offer low-potential detection of bilirubin. The limit of detection is 4 x loT6 M and the response is linear up to 1 X lo-* M. The influence of various experimental variables is described. The surface-to-surface reproducibility is 14% (in terms of relative standard deviation). The fabrication scheme is simple and the response times are short. The prospects of using amperometric bilirubin electrodes for diagnostic work are discussed.

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