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Amperometric biosensor for determination of lactate in sweat

✍ Scribed by Mohammad H. Faridnia; Giuseppe Palleschi; Glenn J. Lubrano; George G. Guilbault

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 461 KB
Volume: 278
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)80082-v

No coin nor oath required. For personal study only.

✦ Synopsis

An amperometric hydrogen peroxide based biosensor has been developed for non-invasive determination of L-lactate. The biosensor utilizes lactate oxidase immobilized between a polycarbonate membrane and a polytetrafluoroethylene (PTFE) blocking membrane to effectively eliinate electrochemical interferences. Both the steady state current and maximum rate of current change were measured. The response times were 2 min and 10 s, respectively. Because of the addition of polycarbonate and PTFE membranes, the linear range of lactate was extended up to 140 mg dl-' and the apparent Michaelis-Menten constant was almost two orders of magnitude higher than that of the free enzyme. The biosensor was applied to the analysis of sweat L-lactate content of healthy subjects during physical exercise.

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