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Mitochondria-Based Amperometric Biosensor for the Determination of L-Glutamic Acid

✍ Scribed by S. T. Girousi; A. A. Pantazaki; A. N. Voulgaropoulos

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 68 KB
Volume: 13
Category: Article
ISSN: 1040-0397
DOI: 10.1002/1521-4109(200103)13:3<243::aid-elan243>3.0.co;2-j

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✦ Synopsis

A mitochondria based amperometric biosensor was developed for the determination of L-glutamic acid. The biosensor was characterized using hexacyanoferrate(III) as the electrochemical mediator. The electrochemical behavior of the redox couple hexacyanoferrate(II)a(III) was improved by incorporating octadecylamine into the enzyme modi®ed carbon paste. L-Glutamic acid was detected at 0.35 V. Linearity was observed in the range 0.4±10 mM of L-glutamic acid (sensitivity 0.189 mAamM, correlation coef®cient 0.997) and in the range 10± 100 mM (sensitivity 0.021 mAamM, correlation coef®cient 0.958). The detection limit was 0.1 mM. The electrode maintains 60 % of its activity after 10 days of use. The bioelectrode was used for the determination of L-glutamate in chicken cube samples.

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