𝔖 Bobbio Scriptorium
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A plasmid cloning vehicle allowing a positive selection for inserted fragments

✍ Scribed by Roberts, Thomas M.; Swanberg, Stephen L.; Poteete, Anthony; Riedel, Gerard; Backman, Keith


Book ID
123427961
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
310 KB
Volume
12
Category
Article
ISSN
0378-1119

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Construction and properties of a new clo
✍ RΓΌther, Ulrich πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 221 KB

pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.