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A novel plasmid vector allowing positive selection for cloned fragments

✍ Scribed by María C. Garrido; JoséL. San Millán; Felipe Moreno

Book ID
109313298
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
293 KB
Volume
34
Category
Article
ISSN
0378-1097

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The vector plasmids pKN001 and pKN80 both contain the EcoRi.C fragment of E.coli phage Mu DNA which codes for a killing function that is efficiently expressed upon transformation into Mu-sensitive bacteria. By in vitro insertion of HindIII fragments at the single HindIII site of pKN80 or of PstI fra