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A plasmid vector allowing positive selection of recombinant plasmids in Streptococcus pneumoniae

✍ Scribed by Prats, Hervé; Martin, Bernard; Pognonec, Philippe; Burger, Anne-Catherine; Claverys, Jean-Pierre

Book ID
122084526
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
689 KB
Volume
39
Category
Article
ISSN
0378-1119

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Construction of an HpaI and HindII plasm
Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids
✍ Schumann, Wolfgang 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 374 KB

The construction of the vector plasmid PKN80 is described, which can be used as HpaI or HindII cloning vehicle with direct selection on transformants harboring hybrid plasmids. pKN80 carries the EcoRI.C fragment of phage Mu DNA coding for a killing function which is efficiently expressed upon transf