𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new substrate for N-acetyl-β-d-glucosaminidase

✍ Scribed by Kazumi Sasamoto; Mikihiko Saito

Book ID
112324502
Publisher
Springer
Year
1988
Tongue
English
Weight
118 KB
Volume
330
Category
Article
ISSN
1618-2650

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A microtiter plate assay for N-acetyl-β-
A microtiter plate assay for N-acetyl-β-d-glucosaminidase using a fluorogenic substrate
✍ S. Linko-Löppönen; M. Mäkinen 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 295 KB

We describe a simple endpoint method for the determination ofN-acetyl-@ghtcosaminidase (NAGase; EC 3.2.1.30). NAGase uses a fluorogenic substrate, 4-methylumbelliferyl-IV-acetyl-@tx-ghtcosaminide, at pH 4.6, liberating the fluorescent 4-methylumbelliferone. The method is reproducible and fast both a

3,4-Dinitrophenyl N-acetyl-β-d-glucosami
3,4-Dinitrophenyl N-acetyl-β-d-glucosaminide, a synthetic substrate for direct spectrophotometric assay of N-acetyl-β-d-glucosaminidase or N-acetyl-β-d-hexosaminidase
✍ Tatsuhiko Yagi; Ryuki Hisada; Hideto Shibata 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 551 KB

## Dinitrophenyl N-acetyl-B-D-glucosaminide (3,4-dnpGlcNAc) was synthesized and proposed as an artificial substrate for N-acetyl-/3-D-glucosaminidase (EC 321.30) and IV -acetyl -@ -D -hexosaminidase (EC 3.2.1.62) (collectively abbreviated as NAGase). 3,4-dnpGlcNAc is water soluble and is fairly st

Purification of endo-N-acetyl-β-d-glucos
Purification of endo-N-acetyl-β-d-glucosaminidase H by substrate-affinity chromatography
✍ Urs F. Greber; Branko Kozulić; Klaus Mosbach 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 815 KB

Endo-N-acetyl-P-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate of Streptomyces plicatus. The key step was substrate-affinity chromatography, which afforded a lOOO-fold purification and yielded a protease-and exoglycosidase-free preparation of Endo H. Pro

Analysis of urinary N-acetyl-β-D-glucosa
Analysis of urinary N-acetyl-β-D-glucosaminidase using 2,4-dinitrophenyl-1-thio N-acetyl-β-D-glucosaminide as the substrate
✍ Magohei Yamada; Toshio Fujita 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 103 KB

## Abstract 2,4‐Dinitrophenyl‐1‐thio __N__‐acetyl‐β‐D‐glucosaminide was examined as a new substrate for analyzing the level of __N__‐acetyl‐β‐D‐glucosaminidase in the urine of patients suffering from renal diseases. The analysis is based on the fact that the substrate, when hydrolyzed in the presen