𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation of modified substrates of N-acetyl-β-D-glucosaminidase

✍ Scribed by V. V. Kolesnikov; M. L. Shul'man; A. Ya. Khorlin

Book ID
112816929
Publisher
Springer
Year
1976
Tongue
English
Weight
353 KB
Volume
25
Category
Article
ISSN
1573-9171

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Purification of endo-N-acetyl-β-d-glucos
Purification of endo-N-acetyl-β-d-glucosaminidase H by substrate-affinity chromatography
✍ Urs F. Greber; Branko Kozulić; Klaus Mosbach 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 815 KB

Endo-N-acetyl-P-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate of Streptomyces plicatus. The key step was substrate-affinity chromatography, which afforded a lOOO-fold purification and yielded a protease-and exoglycosidase-free preparation of Endo H. Pro

3,4-Dinitrophenyl N-acetyl-β-d-glucosami
3,4-Dinitrophenyl N-acetyl-β-d-glucosaminide, a synthetic substrate for direct spectrophotometric assay of N-acetyl-β-d-glucosaminidase or N-acetyl-β-d-hexosaminidase
✍ Tatsuhiko Yagi; Ryuki Hisada; Hideto Shibata 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 551 KB

## Dinitrophenyl N-acetyl-B-D-glucosaminide (3,4-dnpGlcNAc) was synthesized and proposed as an artificial substrate for N-acetyl-/3-D-glucosaminidase (EC 321.30) and IV -acetyl -@ -D -hexosaminidase (EC 3.2.1.62) (collectively abbreviated as NAGase). 3,4-dnpGlcNAc is water soluble and is fairly st