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3,4-Dinitrophenyl N-acetyl-β-d-glucosaminide, a synthetic substrate for direct spectrophotometric assay of N-acetyl-β-d-glucosaminidase or N-acetyl-β-d-hexosaminidase

✍ Scribed by Tatsuhiko Yagi; Ryuki Hisada; Hideto Shibata

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
551 KB
Volume
183
Category
Article
ISSN
0003-2697

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✦ Synopsis

Dinitrophenyl N-acetyl-B-D-glucosaminide

(3,4-dnpGlcNAc) was synthesized and proposed as an artificial substrate for N-acetyl-/3-D-glucosaminidase (EC 321.30) and IV -acetyl -@ -D -hexosaminidase (EC 3.2.1.62) (collectively abbreviated as NAGase). 3,4-dnpGlcNAc is water soluble and is fairly stable in aqueous medium. 3,4-Dinitrophenol released from 3,4-dnp-GlcNAc by NAGase absorbs light of 400 nm at pH near 5, where the activity of urinary NAGase is assayed for diagnostic use. Direct and sensitive spectrophotometric assay of NAGase is, thus, possible using 3,4-dnpGlcNAc as an artificial substrate by monitoring the increase of Ah,,,, due to the release of 3,4-dinitrophenol in a thermostated spectrophotometer.

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