✦ LIBER ✦

Influence of pigments and pH of urine on the determination of N-acetyl-β-D-glucosaminidase activity with 2-methoxy-4-(2′-nitrovinyl)-phenyl-N-acetyl-β-D-glucosaminide

✍ Scribed by Jelena M. Aćimović; Vesna B. Jovanović; Ljuba M. Mandić

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 148 KB
Volume: 19
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.20088

No coin nor oath required. For personal study only.

✦ Synopsis

The influence of urinary pigments and urine pH on the spectrophotometric determination of N-acetyl-b-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2 0nitrovinyl)-phenyl-N-acetyl-b-D-glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2-methoxy-4-(2 0 -nitrovinyl)-phenol (MNP) at 505 nm. It was shown that besides the maximum (I) in the range of 350-360 nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380-460 nm. With the increase of pH, maximum II was shifted toward higher wavelengths and contributed to MNP absorption (5-90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495-400 nm) with increasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed.

📜 SIMILAR VOLUMES

Analysis of urinary N-acetyl-β-D-glucosa

Analysis of urinary N-acetyl-β-D-glucosaminidase using 2,4-dinitrophenyl-1-thio N-acetyl-β-D-glucosaminide as the substrate

✍ Magohei Yamada; Toshio Fujita 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 103 KB

## Abstract 2,4‐Dinitrophenyl‐1‐thio __N__‐acetyl‐β‐D‐glucosaminide was examined as a new substrate for analyzing the level of __N__‐acetyl‐β‐D‐glucosaminidase in the urine of patients suffering from renal diseases. The analysis is based on the fact that the substrate, when hydrolyzed in the presen

ChemInform Abstract: New Effective Synth

ChemInform Abstract: New Effective Synthesis of (N-Acetyl- and N-Stearoyl-2-amino-2-deoxy-β-D-glucopyranosyl)- (1→4)-N-acetylnormuramoyl-L-2-aminobutanoyl-D-isoglutamine, Analogues of GMDP with Immunopotentiating Activity.

✍ M. LEDVINA; D. ZYKA; J. JEZEK; T. TRNKA; D. SAMAN 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 31 KB 👁 1 views

IN FETUSES WITH ISOLATED HYDRONEPHROSIS,

IN FETUSES WITH ISOLATED HYDRONEPHROSIS, URINARY β2-MICROGLOBULIN AND N-ACETYL-β-D-GLUCOSAMINIDASE (NAG) HAVE A LIMITED ROLE IN THE PREDICTION OF POSTNATAL RENAL FUNCTION

✍ BEATRICE M. G. TASSIS; LAURA TRESPIDI; AMEDEA S. TIRELLI; LUISA BOCCONI; CINZIA 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 522 KB

Seventy-one fetal urine samples were taken from the bladder or renal pelvis of 33 fetuses at 13-36 weeks' gestation with a diagnosis of urinary tract anomaly. Severe isolated hydronephrosis in the absence of an enlarged bladder was the indication for sampling in 12/33 fetuses (26 samples), who were

Influence of 1-dodecylazacycloheptan-2-o

Influence of 1-dodecylazacycloheptan-2-one (azone) on the topical therapy of cutaneous herpes simplex virus type 1 infection in hairless mice with 2′,3′-di-o-acetyl-9-β-D-arabinofuranosyladenine and 5′-O-vaieryl-9-β-D-arabinofuranosyladenine

✍ W. M. Shannon; L. Westbrook; W. I. Higuchi; K. Sugibayashi; D. C. Baker; S. D. K 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 447 KB