𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A practical new assay for determining N-acetyl-β-D-glucosaminidase activity in urine

✍ Scribed by Klaus Jung; Friedrich Priem

Book ID
115827274
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
181 KB
Volume
203
Category
Article
ISSN
0009-8981

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A microtiter plate assay for N-acetyl-β-
A microtiter plate assay for N-acetyl-β-d-glucosaminidase using a fluorogenic substrate
✍ S. Linko-Löppönen; M. Mäkinen 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 295 KB

We describe a simple endpoint method for the determination ofN-acetyl-@ghtcosaminidase (NAGase; EC 3.2.1.30). NAGase uses a fluorogenic substrate, 4-methylumbelliferyl-IV-acetyl-@tx-ghtcosaminide, at pH 4.6, liberating the fluorescent 4-methylumbelliferone. The method is reproducible and fast both a

3,4-Dinitrophenyl N-acetyl-β-d-glucosami
3,4-Dinitrophenyl N-acetyl-β-d-glucosaminide, a synthetic substrate for direct spectrophotometric assay of N-acetyl-β-d-glucosaminidase or N-acetyl-β-d-hexosaminidase
✍ Tatsuhiko Yagi; Ryuki Hisada; Hideto Shibata 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 551 KB

## Dinitrophenyl N-acetyl-B-D-glucosaminide (3,4-dnpGlcNAc) was synthesized and proposed as an artificial substrate for N-acetyl-/3-D-glucosaminidase (EC 321.30) and IV -acetyl -@ -D -hexosaminidase (EC 3.2.1.62) (collectively abbreviated as NAGase). 3,4-dnpGlcNAc is water soluble and is fairly st