𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new standard reference material for column evaluation in reversed-phase liquid chromatography

✍ Scribed by Lane C. Sander; Stephen A. Wise

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
500 KB
Volume
26
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A new Standard Reference Material (SRM) for use in characterizing general aspects of liquid chromatographic column performance is described. SRM 870 Column Performance Test Mixture for Liquid Chromatography contains five components: uracil, toluene, ethylbenzene, quinizarin, and amitriptyline, and is intended to provide indications of column void volume, hydrophobicity, methylene selectivity, activity toward organic bases, and activity toward metal chelators. The test is performed under neutral, buffered conditions. The measurement of retention and peak asymmetry factors for the components facilitates column classification. It is anticipated that this material will be useful (1) in column selection during method development, (2) as a control material to monitor column performance over time, and (3) in quality control in column manufacturing.

πŸ“œ SIMILAR VOLUMES

A trapping column for the coupling of re
A trapping column for the coupling of reversed-phase liquid chromatography and capillary gas chromatography
✍ Vreuls, Jolan J. ;Goudriaan, Vincent P. ;Th, Udo A. ;de Jong, Gerhardus J. πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 611 KB

## Abstract A new technique for coupling reversed‐phase liquid chromatography (RPLC) with gas chromatography is described. A fraction eluting from an RPLC column is trapped on a short column packed with polymeric adsorbent. After the mobile phase has been displaced with water, the analytes are deso

Evaluation of etched borosilicate glass
Evaluation of etched borosilicate glass capillary columns in reversed phase open tubular liquid chromatography
✍ Daniel M. Dohmeier; James W. Jorgenson πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 506 KB

## Abstract The performance of etched borosilicate capillary columns with a bonded octadecyl stationary phase has been examined. Plate heights for three test compounds in columns with inner diameters between 6.5 and 14.6 ΞΌm were measured as a function of mobile phase velocity. The reduced plate hei

An evaluation of the robustness of the T
An evaluation of the robustness of the Tanaka characterization protocol for reversed-phase liquid chromatography columns
✍ Patrik Petersson; Melvin R. Euerby πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 362 KB

## Abstract The robustness of the popular β€˜Tanaka’ LC column characterization protocol has been evaluated by the statistical tools of reduced factorial design, multiple linear regression and principal component analysis. These have shown that in order to obtain reliable and reproducible results, it