✦ LIBER ✦

Evaluation of etched borosilicate glass capillary columns in reversed phase open tubular liquid chromatography

✍ Scribed by Daniel M. Dohmeier; James W. Jorgenson

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 506 KB
Volume: 3
Category: Article
ISSN: 1040-7685
DOI: 10.1002/mcs.1220030404

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✦ Synopsis

Abstract

The performance of etched borosilicate capillary columns with a bonded octadecyl stationary phase has been examined. Plate heights for three test compounds in columns with inner diameters between 6.5 and 14.6 μm were measured as a function of mobile phase velocity. The reduced plate height vs. reduced velocity curves were then fit to the Golay equation using nonlinear regression analysis. It was found that in addition to longitudinal diffusion in the mobile phase, longitudinal diffusion in the stationary phase was a significant contributor to broadening at low mobile phase flow rates. At higher flow rates, resistance to mass transfer in the stagnant mobile phase in the porous glass produced by the etching procedure became measurable.

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