๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Preparation of reversed phase open tubular liquid chromatographic columns from borosilicate glass

โœ Scribed by Robert L. St. Claire III; Daniel M. Dohmeier; James W. Jorgenson

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
570 KB
Volume
3
Category
Article
ISSN
1040-7685

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

The preparation of open tubular liquid chromatographic columns with small inner diameters (โ‰ค 20 ฮผm) from Pyrex 7740 borosilicate glass is described. The surface area of the inner wall of the capillary is increased by etching with a series of acidic and basic solutions. Dimethyloctadecylchlorosilane is then reacted with the column wall to create a bonded stationary phase layer. The resulting columns exhibit good retention and high efficiency. The effects of each step in the etching process are examined and the silanization reaction is optimized.

๐Ÿ“œ SIMILAR VOLUMES

Evaluation of etched borosilicate glass
Evaluation of etched borosilicate glass capillary columns in reversed phase open tubular liquid chromatography
โœ Daniel M. Dohmeier; James W. Jorgenson ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 506 KB

## Abstract The performance of etched borosilicate capillary columns with a bonded octadecyl stationary phase has been examined. Plate heights for three test compounds in columns with inner diameters between 6.5 and 14.6 ฮผm were measured as a function of mobile phase velocity. The reduced plate hei

New method for the preparation of small
New method for the preparation of small diameter columns with polymeric stationary phases for open tubular liquid chromatography
โœ Dluzneski, P. R. ;Jorgenson, J. W. ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 461 KB ๐Ÿ‘ 1 views

Thepurposeof this report is tointroduceanewmethodforusein coating polymeric stationary phase films on the inside wall of small bore diameter fused silica capillary tubing. This technique is being developed for use in fabricating capillary columns for open tubular liquid chromatography.