A new positive/negative selectable marke
โ
You-Tzung Chen; Allan Bradley
๐
Article
๐
2000
๐
John Wiley and Sons
๐
English
โ 152 KB
## A novel positive/negative selection cassette, puโฌtk, was generated. puโฌtk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (โฌTk). Murine embryonic stem (ES) cells transfected with puโฌtk become res