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Escherichia coli gpt as a positive and negative selectable marker in embryonal stem cells

✍ Scribed by K.J. Spring; J.S. Mattick; R.H. Don


Book ID
113263084
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
452 KB
Volume
1218
Category
Article
ISSN
0167-4781

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## A novel positive/negative selection cassette, pu⌬tk, was generated. pu⌬tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (⌬Tk). Murine embryonic stem (ES) cells transfected with pu⌬tk become res