A new positive/negative selectable marke
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You-Tzung Chen; Allan Bradley
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Article
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2000
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John Wiley and Sons
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English
β 152 KB
## A novel positive/negative selection cassette, puβ¬tk, was generated. puβ¬tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (β¬Tk). Murine embryonic stem (ES) cells transfected with puβ¬tk become res