A new positive/negative selectable marker, puΔtk, for use in embryonic stem cells

✍ Scribed by You-Tzung Chen; Allan Bradley

• Book ID: 101394781
• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 152 KB
• Volume: 28
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/1526-968x(200009)28:1<31::aid-gene40>3.0.co;2-k

✦ Synopsis

A novel positive/negative selection cassette, pu⌬tk, was generated. pu⌬tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (⌬Tk). Murine embryonic stem (ES) cells transfected with pu⌬tk become resistant to puromycin and sensitive to 1-(-2-deoxy-2-fluoro-1-␤-D-arabino-furanosyl)-5-iodouracil (FIAU). Unlike other HSV1

tk transgenes, pu⌬tk is readily transmitted through the male germ line. Thus pu⌬tk is a convenient positive/ negative selectable marker that can be widely used in many ES cell applications. genesis 28:31-35, 2000.