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A New Positive/Negative Selection Scheme for Precise BAC Recombineering

โœ Scribed by Shuwen Wang; Yuanjun Zhao; Melanie Leiby; Jiyue Zhu


Book ID
107581689
Publisher
Humana Press Inc
Year
2009
Tongue
English
Weight
333 KB
Volume
42
Category
Article
ISSN
1073-6085

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## A novel positive/negative selection cassette, puโŒฌtk, was generated. puโŒฌtk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (โŒฌTk). Murine embryonic stem (ES) cells transfected with puโŒฌtk become res