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A new sample applicator for isoelectric focusing in horizontal polyacrylamide gels

✍ Scribed by Prof. Dr. Klaus Altland; Pia Becher


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
507 KB
Volume
9
Category
Article
ISSN
0173-0835

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A specific stain for Ξ±-glucosidases in i
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a-Glucosidases separated on isoelectric focusing gels can be localized by a two-step staining procedure. First, bromonaphthyl-cu-D-glucoside is cleaved by the a-glucosidases in the gel; then, the released bromonaphthol is precipitated with Fast Blue B salt. The procedure is specific for a-glucosidas